TwistAmp Basic RT试剂盒

更新时间：2017-02-22 13:24:54

价格：请来电询价

品牌:TwistDX

型号:Basic RT

货号:TABSRT01KIT

联系电话：13810792952

联系手机：13810792952

联系人：刘经理

让卖家联系我

详细介绍

TABSRT01KIT

TwistAmp™ Basic RT

用于逆转录RPA反应

产品描述：

TwistAmp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp Basic用于逆转录反应，CDNA快速扩增. 除基本组分外, 还加有通用的逆转录酶，但不含Rnase抑制剂。

Basic Information

RPA

1) Primers must be 30-35 bases

2) Works best at constant temperature (40-42°C)

3) Amplicons of 80-400bp are

Set-up (single-plex)1

1) Prepare reaction mix in 1.5ml tube:

Primer A (10μM) 2.4 μl

Primer B (10μM) 2.4 μl

Rehydration Buffer 29.5 μl

Template,

RNase Inhibitor and dH2O 13.2 μl

(Total Volume 47.5 μl)

Vortex and spin briefly

2) Add reaction mix to freeze-dried reaction. Pipette to mix.

3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.

4) Incubate at 40-42°C for 20-40 minutes.

4b) For low template copy number,

remove strip after 5-7 minutes, invert

vigorously 8-10 times to mix & spin

briefly, replace in heating device.

5) After 20-40 minutes, clean amplicons before running on agarose gels.

WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS

MAGNESIUM IS ADDED.

产品分类

站内搜索