TwistAmp exo RT试剂盒

更新时间：2017-02-22 13:31:28

价格：请来电询价

品牌:TwistDX

型号:exo RT

货号:TAEXORT02KIT

联系电话：13810792952

联系手机： 13810792952

联系人：刘经理

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详细介绍

TAEXORT02KIT

TwistAmp™ exo RT

荧光检测探针法RT-RPA

产品描述：

TwistAmp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp exo RT荧光检测探针法RT-RPA. 除基本组分外, 外加通用的逆转录酶，但不含Rnase抑制剂。

Basic Information

RPA

1) Primers must be 30-35 bases

2) Works best at constant temperature (40°C)

3) Amplicons of 80-400bp are preferred

4) TwistAmp® exo Probe required - see overleaf

Set-up (single-plex)1

1) Prepare reaction mix in 1.5ml tube:

Primer A (10μM) 2.1 μl

Primer B (10μM) 2.1 μl

TwistAmp® exo Probe (10μM) 0.6 μl

Rehydration Buffer 29.5 μl

Template,

RNase Inhibitor and dH2O 13.2 μl

(Total Volume 47.5 μl)

2) Add reaction mix to freeze-dried reaction. Pipette to mix.

3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.

4) Place strip in Twista® and start run: 40°C, 20 minutes.

5) Remove strip after 5-7 minutes, invert vigorously 8-10 times to mix & spin briefly,replace in Twista.

WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS

MAGNESIUM IS ADDED.

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